Bacquian, Megan Cate G., Baluyot, Ashley Rubiel S., Cenidoza, Daphne Ashlie C., Delantar, Patricia Kaye P., German, Samantha Ysabel G. 4 0
Synergistic antibacterial potential of salvinia auriculata (Butterfly Fern) and ludwigia perennis (Perennial Water Primrose) leaf methanolic extract against staphylococcu aureus and escherichia coli 6 6 Bacquian, Megan Cate G., Baluyot, Ashley Rubiel S., Cenidoza, Daphne Ashlie C., Delantar, Patricia Kaye P., German, Samantha Ysabel G. - - - 102 pages - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (BS Biology major in Medical Biology) - Pamantasan ng Lungsod ng Maynila, 2024
5
ABSTRACT: Antimicrobial drug resistance is a major global challenge, with rising costs for finding effective drugs and a declining rate of new drug discoveries. This study aims to evaluate the synergistic antibacterial potential of Salvinia auriculata and Ludwigia perennis methanolic extracts alongside positive controls, Psidium guajava and Ocimum gratissimum, against Staphylococcus aureus and Escherichia coli. Minimum inhibitory concentration (MIC) (from concentrations, 400mg/ml - 0.195mg/ml), minimum bactericidal concentration (MBC), and disc diffusion assays were utilized to determine the antibacterial activities of individual and combined plant extracts. The MIC values for individual extracts against E. coli and S. aureus were 12.5 and 6.25 mg/ml (S. auriculata), and 6.25 mg/ml and 300mg/ml (L. perennis) whilst combined extracts, SL 1:3 and 3:1 had the lowest MIC against E. coli (0.781 mg/ml) and SL 3:1 against S. aureus (0.390625 mg/ml). This showed a highly significant difference in S. auriculata (p = 0.003) and the combined S. auriculata and L. perennis (p = 0.001) compared to the positive control. MBC results indicated bactericidal activity for most extracts and combinations against E. coli but only moderate efficacy against S. aureus. The combined plant extracts of S. auriculata and L. perennis demonstrated superior inhibitory activity compared to individual extracts, especially against E. coli, with mean inhibition zones of 10± 0.4 mm to 9.6 ± 1.5 mm for SL 3:1 and 1:3 ratio, respectively. It revealed significantly different zones of inhibition, with most ratios having a p-value < 0.05. Synergistic effects were observed in SL 1:1, 1:3, and 3:1, (FICi = 0.375 to 0.188) against E. coli, indicating potential for enhanced antibacterial activity. The synergism of S. auriculata and L. perennis is of great value as natural antibacterial agents and the study underscores the importance of exploring plant extract combinations to combat antimicrobial resistance.
5
2 = =
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/ 2
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Synergistic antibacterial potential of salvinia auriculata (Butterfly Fern) and ludwigia perennis (Perennial Water Primrose) leaf methanolic extract against staphylococcu aureus and escherichia coli 6 6 Bacquian, Megan Cate G., Baluyot, Ashley Rubiel S., Cenidoza, Daphne Ashlie C., Delantar, Patricia Kaye P., German, Samantha Ysabel G. - - - 102 pages - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (BS Biology major in Medical Biology) - Pamantasan ng Lungsod ng Maynila, 2024
5
ABSTRACT: Antimicrobial drug resistance is a major global challenge, with rising costs for finding effective drugs and a declining rate of new drug discoveries. This study aims to evaluate the synergistic antibacterial potential of Salvinia auriculata and Ludwigia perennis methanolic extracts alongside positive controls, Psidium guajava and Ocimum gratissimum, against Staphylococcus aureus and Escherichia coli. Minimum inhibitory concentration (MIC) (from concentrations, 400mg/ml - 0.195mg/ml), minimum bactericidal concentration (MBC), and disc diffusion assays were utilized to determine the antibacterial activities of individual and combined plant extracts. The MIC values for individual extracts against E. coli and S. aureus were 12.5 and 6.25 mg/ml (S. auriculata), and 6.25 mg/ml and 300mg/ml (L. perennis) whilst combined extracts, SL 1:3 and 3:1 had the lowest MIC against E. coli (0.781 mg/ml) and SL 3:1 against S. aureus (0.390625 mg/ml). This showed a highly significant difference in S. auriculata (p = 0.003) and the combined S. auriculata and L. perennis (p = 0.001) compared to the positive control. MBC results indicated bactericidal activity for most extracts and combinations against E. coli but only moderate efficacy against S. aureus. The combined plant extracts of S. auriculata and L. perennis demonstrated superior inhibitory activity compared to individual extracts, especially against E. coli, with mean inhibition zones of 10± 0.4 mm to 9.6 ± 1.5 mm for SL 3:1 and 1:3 ratio, respectively. It revealed significantly different zones of inhibition, with most ratios having a p-value < 0.05. Synergistic effects were observed in SL 1:1, 1:3, and 3:1, (FICi = 0.375 to 0.188) against E. coli, indicating potential for enhanced antibacterial activity. The synergism of S. auriculata and L. perennis is of great value as natural antibacterial agents and the study underscores the importance of exploring plant extract combinations to combat antimicrobial resistance.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/