De Guzman, Ramon Carlos R., Plete, Rianne Victoria C., Siruno, Alleah Nicole S. 4 0
Synergistic anti-angiogenic effect of maidenhair tree (Ginkgo biloba L.) and sabah snake grass (Clinicanthus nutans Burm F. Lindau) leaf crude methanolic extract on chick embryo in ovo chorioallantoic membrane (CAM) assay 6 6 De Guzman, Ramon Carlos R., Plete, Rianne Victoria C., Siruno, Alleah Nicole S. - - - 165 pages - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (BS Biology major in Medical Biology) - Pamantasan ng Lungsod ng Maynila, 2024
5
ABSTRACT: The study investigated the synergistic anti-angiogenic activity of maidenhair tree (Ginkgo biloba L.) and Sabah snake grass (Clinacanthus nutans Burm f. Lindau) leaf crude methanolic extract utilizing chick embryos via in ovo chorioallantoic membrane assay. Brine shrimp lethality assay was utilized to get the median lethal concentration or LC50 of the experimental treatments. For quantitative analysis, Fiji/ImageJ software was employed. The study employs both macroscopic evaluation using a Semi-Quantitative Scoring System and quantitative analysis via Fiji/ImageJ software to measure vessel length and vessel diameter, to further assess synergistic and antagonistic interactions of the combination extract across different concentrations. Results indicate a synergistic effect at 100% concentration, notably reducing average total vessel length, while antagonistic interactions are observed concerning average vessel diameter. G. biloba alone demonstrates significant anti-angiogenic effects, particularly at higher concentrations, attributed to the presence of phytochemicals inhibiting angiogenic pathways. Semi-quantitative evaluation suggests strong anti-angiogenic effects for the 1:1 combination at 25% concentration, albeit potentially influenced by human error and bias upon comparison with the positive control Bevacizumab which yielded non-statistically significant results. The findings underscore the potent anti-angiogenic potential of G. biloba, especially at higher concentrations, while the 1:1 combination showed concentration-dependent responses, meriting further exploration for therapeutic applications. Addressing limitations and pursuing future research directions outlined herein can advance the development of novel anti-angiogenic agents and explore synergistic combinatory therapies.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
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/ 2
/ 2
/
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Synergistic anti-angiogenic effect of maidenhair tree (Ginkgo biloba L.) and sabah snake grass (Clinicanthus nutans Burm F. Lindau) leaf crude methanolic extract on chick embryo in ovo chorioallantoic membrane (CAM) assay 6 6 De Guzman, Ramon Carlos R., Plete, Rianne Victoria C., Siruno, Alleah Nicole S. - - - 165 pages - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (BS Biology major in Medical Biology) - Pamantasan ng Lungsod ng Maynila, 2024
5
ABSTRACT: The study investigated the synergistic anti-angiogenic activity of maidenhair tree (Ginkgo biloba L.) and Sabah snake grass (Clinacanthus nutans Burm f. Lindau) leaf crude methanolic extract utilizing chick embryos via in ovo chorioallantoic membrane assay. Brine shrimp lethality assay was utilized to get the median lethal concentration or LC50 of the experimental treatments. For quantitative analysis, Fiji/ImageJ software was employed. The study employs both macroscopic evaluation using a Semi-Quantitative Scoring System and quantitative analysis via Fiji/ImageJ software to measure vessel length and vessel diameter, to further assess synergistic and antagonistic interactions of the combination extract across different concentrations. Results indicate a synergistic effect at 100% concentration, notably reducing average total vessel length, while antagonistic interactions are observed concerning average vessel diameter. G. biloba alone demonstrates significant anti-angiogenic effects, particularly at higher concentrations, attributed to the presence of phytochemicals inhibiting angiogenic pathways. Semi-quantitative evaluation suggests strong anti-angiogenic effects for the 1:1 combination at 25% concentration, albeit potentially influenced by human error and bias upon comparison with the positive control Bevacizumab which yielded non-statistically significant results. The findings underscore the potent anti-angiogenic potential of G. biloba, especially at higher concentrations, while the 1:1 combination showed concentration-dependent responses, meriting further exploration for therapeutic applications. Addressing limitations and pursuing future research directions outlined herein can advance the development of novel anti-angiogenic agents and explore synergistic combinatory therapies.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/