Diane Thaise C. Africano. 4 0
MIicrobial generation of electricity using enterobacter / 6 6 Diane Thaise C. Africano. - - - 26 pp. 28 cm. - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (Bachelor of Science in Chemistry) - Pamantasan ng Lungsod ng Maynila, 2003.
5
ABSTRACT: A major trend in energy demand has been a dramatic increase in the use of electricity. People have continually demanded additional goods and services that are energy intensive, that is, have high energy requirement. Because of these, current demands constantly growing nations and their industries. We must explore other viable means to generate energy-tap into technologies that have tremendous potential but have received so little support and notice. One such option is harnessing the bioelectricity produced by microorganisms. This use of a living beauty to supply significant amounts of electricity holds infinite possibilities-the production of seemingly endless energy at relatively low input costs. With the analysis reports that facultative anaerobic bacteria, which can survive with or without air has the ability to convert waste to methane gas, it is the purpose of this study to investigate its ability to generate electricity. It also aims to (1) provide possible alternative culture medium such as knorr with banana, pineapple with urea, and melon with (NH4)3PO4 aside from the nutrient broth commonly used in the laboratories and (2) to determine the optimum pH for each culture medium that will be suitable for the maximum generation of electrical voltage. The experimental procedure consists of the preparation of the ferricyanide solution, preparation of knorr with banana, pineapple with urea and melon with (NH4)3PO4 as the culture media to be used. Inoculation into Petri dish, measurement of cell density using spectrophotometer, and determination of voltage. The series of experiments conducted entail recording of the voltage readings that would be important in determining the trends and evaluating the adaptability of Enterobacter in certain culture media. The culture media used were Melon with (NH4)3PO4, Pineapple with Urea, and Banana with knorr beef cube. The Enterobacter grows best and produced the optimum or highest voltage in Melon with (NH4)3PO4 at pH5, while in Pineapple with Urea, and in Banana with knorr beef cube, at pH7. The researcher also found out that among the three culture media used, at pH 5, Melon with (NH4)3PO4 gave the highest or optimum voltage, while in pH6 and pH 7, Pineapple with Urea yielded the highest voltage. In overall reaction, I can say that the voltage produced by the different culture media is directly proportional to its growth curve. The higher the growth curve, the higher the voltage produced. However, the electrical voltage produced was not suffiecient for commercial use.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/
MIicrobial generation of electricity using enterobacter / 6 6 Diane Thaise C. Africano. - - - 26 pp. 28 cm. - - - - - . - . - 0 . - . - 0 .
Undergraduate Thesis: (Bachelor of Science in Chemistry) - Pamantasan ng Lungsod ng Maynila, 2003.
5
ABSTRACT: A major trend in energy demand has been a dramatic increase in the use of electricity. People have continually demanded additional goods and services that are energy intensive, that is, have high energy requirement. Because of these, current demands constantly growing nations and their industries. We must explore other viable means to generate energy-tap into technologies that have tremendous potential but have received so little support and notice. One such option is harnessing the bioelectricity produced by microorganisms. This use of a living beauty to supply significant amounts of electricity holds infinite possibilities-the production of seemingly endless energy at relatively low input costs. With the analysis reports that facultative anaerobic bacteria, which can survive with or without air has the ability to convert waste to methane gas, it is the purpose of this study to investigate its ability to generate electricity. It also aims to (1) provide possible alternative culture medium such as knorr with banana, pineapple with urea, and melon with (NH4)3PO4 aside from the nutrient broth commonly used in the laboratories and (2) to determine the optimum pH for each culture medium that will be suitable for the maximum generation of electrical voltage. The experimental procedure consists of the preparation of the ferricyanide solution, preparation of knorr with banana, pineapple with urea and melon with (NH4)3PO4 as the culture media to be used. Inoculation into Petri dish, measurement of cell density using spectrophotometer, and determination of voltage. The series of experiments conducted entail recording of the voltage readings that would be important in determining the trends and evaluating the adaptability of Enterobacter in certain culture media. The culture media used were Melon with (NH4)3PO4, Pineapple with Urea, and Banana with knorr beef cube. The Enterobacter grows best and produced the optimum or highest voltage in Melon with (NH4)3PO4 at pH5, while in Pineapple with Urea, and in Banana with knorr beef cube, at pH7. The researcher also found out that among the three culture media used, at pH 5, Melon with (NH4)3PO4 gave the highest or optimum voltage, while in pH6 and pH 7, Pineapple with Urea yielded the highest voltage. In overall reaction, I can say that the voltage produced by the different culture media is directly proportional to its growth curve. The higher the growth curve, the higher the voltage produced. However, the electrical voltage produced was not suffiecient for commercial use.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/