Don Karlo R. Nabus. 4 0
Anti-angiogenic potential of dog's tongue (pseudelephantopus spicatus (Juss.) Rohr.) plant extract in chick choroallantoic membrane / 6 6 Don Karlo R. Nabus. - - - 70 pp. 28 cm. - - - - - . - . - 0 . - . - 0 .
Thesis:(B.S. Biology) Pamantasan ng Lungsod ng Maynila, 2017.
5
ABSTRACT: Blood vessel formation is an important component for an organism to survive. Angiogenesis must be maintained in optimal amounts. Too much or less amount of blood vessel may lead to disease and complications such as tumors. The researcher used chick chorioallantoic membrane (CAM) model to demonstrate the inhibition of blood vessel formation with the use of Pseudelephantopus spicatus plant extract. This research focused on the angiogenic activity of P. Spicatus plant extract of varying concentrations (84.75, 63.75, 42.75, and 21.19mg/ml) compared to Camellia sinensis which served as the positive control, while ethanol served as the negative control in this experiment. The extracts were applied on the eight day of incubation and were observed two days after the application. The angiogenic inhibition was assessed according to three different criteria: vessel density, vessel length, and branching point. Results showed that 84.75 mg/ml of P. Spicatus showed the highest percent inhibition (25.91%). The inhibition recorded was dose dependent with the highest concentration being at the the top of the list. Wide range of effectivity was observed depending on the concentration used. No significant difference was noted between 84.75 mg/ml and the positive control (p=0.014, 0.005<0.050). Generally speaking, the positive control and the 84.75 mg/ml of P. Spicatusare of the same level of effectivity. No significant difference was also calculated within the varying concentrations of the treatment group making all the concentrations of equal effectivity (p= 0.581, 0.561<0.050). These results imply that P. Spicatus could be a source of anti-angiogenic compunds and can be studied further.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/
Anti-angiogenic potential of dog's tongue (pseudelephantopus spicatus (Juss.) Rohr.) plant extract in chick choroallantoic membrane / 6 6 Don Karlo R. Nabus. - - - 70 pp. 28 cm. - - - - - . - . - 0 . - . - 0 .
Thesis:(B.S. Biology) Pamantasan ng Lungsod ng Maynila, 2017.
5
ABSTRACT: Blood vessel formation is an important component for an organism to survive. Angiogenesis must be maintained in optimal amounts. Too much or less amount of blood vessel may lead to disease and complications such as tumors. The researcher used chick chorioallantoic membrane (CAM) model to demonstrate the inhibition of blood vessel formation with the use of Pseudelephantopus spicatus plant extract. This research focused on the angiogenic activity of P. Spicatus plant extract of varying concentrations (84.75, 63.75, 42.75, and 21.19mg/ml) compared to Camellia sinensis which served as the positive control, while ethanol served as the negative control in this experiment. The extracts were applied on the eight day of incubation and were observed two days after the application. The angiogenic inhibition was assessed according to three different criteria: vessel density, vessel length, and branching point. Results showed that 84.75 mg/ml of P. Spicatus showed the highest percent inhibition (25.91%). The inhibition recorded was dose dependent with the highest concentration being at the the top of the list. Wide range of effectivity was observed depending on the concentration used. No significant difference was noted between 84.75 mg/ml and the positive control (p=0.014, 0.005<0.050). Generally speaking, the positive control and the 84.75 mg/ml of P. Spicatusare of the same level of effectivity. No significant difference was also calculated within the varying concentrations of the treatment group making all the concentrations of equal effectivity (p= 0.581, 0.561<0.050). These results imply that P. Spicatus could be a source of anti-angiogenic compunds and can be studied further.
5
2 = =
2
2 --0------
6 --0-- 2 --------
0 2 --
--20------
--------20--
--------20--
----2
/ 2
/ 2
/
/