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Production of Peptone from Chicken Feathers 6 6 - - - - - - - - . - . - 0 . - . - 0 .

ABSTRACT : Peptones are the product of a protein hydrolysis, which serve as the main nutrient source for bacteria in a culture media. This study aimed to provide a locally feasible process for peptone production. Optimization results for Phase 1 showed that a temperature of 90C, a digestion time of 4 hours and a ratio 0.06g feathers/mL produced a yield of 50.6%. Statistical analysis showed that E. coli growth on the laboratory - produced peptone is significantly greater than the growth realized on commercial peptone and plate count agar. Purification, dying and characterization techniques were integrated into the existing process for Phase 2 to obtain peptones with better commercial quality. The maximum yield obtained was 30 g peptone per 100 g feathers. Freeze-dried powder from the purified hydrolysates had reduced odor and moisture as compared to the vacuum dried peptone . The vacuum-filtered batch also approximated the physical characteristics of the standard peptone hyfrolysate. Performance testing for Phase 2 showed increased support for bacterial growth.





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Peptone Production ------Nutrient Media----

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