| 000 -LEADER |
|---|
| fixed length control field |
02825nam a22002417a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
ft8644 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20251013160746.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
251013b ||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
engtag |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QD146 T37 2025 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
. |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tarroza, Paul Justin D.; Malihan, Jhanna Lea S.; Santiago, Abegail R. |
| 245 ## - TITLE STATEMENT |
|---|
| Title |
Comparative determination of vitamin C on non-FDA-approved calamansi fruit juices using UV-VIS spectroscopy and HPLC and its general microbiological qualities |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Manila: |
| Name of producer, publisher, distributor, manufacturer |
PLM, |
| Date of production, publication, distribution, manufacture, or copyright notice |
c2025 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Other physical details |
Undergraduate Thesis: (Bachelor of Science in Chemistry) - Pamantasan ng Lungsod ng Maynila, 2025 |
| 336 ## - CONTENT TYPE |
|---|
| Source |
text |
| Content type term |
text |
| Content type code |
text |
| 337 ## - MEDIA TYPE |
|---|
| Source |
unmediated |
| Media type term |
unmediated |
| Media type code |
unmediated |
| 338 ## - CARRIER TYPE |
|---|
| Source |
volume |
| Carrier type term |
volume |
| Carrier type code |
volume |
| 505 ## - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
ABSTRACT: Vitamin C in fruit juices is essential in collagen synthesis, absorption of nonheme iron from foods, and counters high oxidative stress. The Philippine fruit juice market is growing due to urbanization and health trends. However, unregistered juices pose health risks due to lack of quality control. Accurate vitamin C quantification methods like UV-Visible spectroscopy and High-Pressure Liquid Chromatography are crucial. This research study aimed to determine the mean vitamin C concentration in non-FDA-approved calamansi fruit juices using UV-Vis spectrophotometry and High-Performance Liquid Chromatography. It also sought to assess any significant differences in vitamin C concentration among three fruit juice samples, compare the HPLC and UV- Vis methods and evaluate the microbiological quality of the juices by determining the Aerobic Plate Count, E coli, total coliform, and yeast and mold counts. The UV-Vis method used an indirect approach with potassium permanganate (KMnO4) for standard calibration and the analysis of fruit juices. The vitamin C concentration was higher with UV-Vis compared to HPLC. This difference was due to various factors affecting absorbance readings, such as reducing sugars, sample color, acids in the sample matrix, and instrumental and personal error. For HPLC, a metaphosphoric acid solution was used as the solvent for the fruit juice samples. Statistical analysis of the results from both UV-Vis and HPLC methods showed significant differences in vitamin C concentrations among the juice samples with fruit juice 2 showing no significant difference. While the HPLC method revealed significant differences among all three juices, the UV-Vis method showed no significant differences. Microbiological tests indicated that all juice samples met safety standards for consumption, confirming that they were microbiologically safe, with acceptable levels of APC, E. coli, total coliform, and yeast and mold counts.<br/> |
| 526 ## - STUDY PROGRAM INFORMATION NOTE |
|---|
| Classification |
Filipiniana |
| 655 ## - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
academic writing |
| 942 ## - ADDED ENTRY ELEMENTS |
|---|
| Source of classification or shelving scheme |
|
| Item type |
Thesis/Dissertation |