Antimicrobial potential of sarsalida (glinus opposittifolius) plant crude saponin against staphylococcus aureus, escherichia coli, and aspergillus niger. 6

Thesis : (B.S. Biology)-Pamantasan ng Lungsod ng Maynila, 2006. 56

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ABSTRACT: The study focuses on the Antimicrobial Potential of Sarsalida (Glinus oppositifolius) Plant Crude Saponin Against Stphylococcus aureus, Escherichia coli, and Aspergillus niger. The air-dried plant of sarsalida (Glinus oppositifolius)afforded methaholic crude extract that were used in the phytochemical analysis and where crude saponin were extracted. The phytochemical analysis revealed the presence of saponin, cardiac glycosides and tannins and the absence of alkaloids, flavonoids, anthraquinones, fats and oils plant constituents. Solvent system was used in the isolation process of the yield crude saponin extract. A total of 6 solvent systems were tried out and the best separation of constituent was seen on the n-BuOH-AcOH-H2O 5:1:4 v/v ratio. The crude saponin extract used was able to isolate a semi-pure saponin comnpound by preparative thin layer chromatography with silica gel G. In the antimicrobial assay, indicated the low antimicrobial activities against Staphylococcus aureus, Escherichia coli and no inhibition against Aspergillus niger at varied concentrations. Results from the statistical analysis showed that there was a significant difference on the varying concentrations among the microorganisms. In Staphylococcus aureus, the concentrations were significantly difference on the 50%, 75% and 100% and there was no significant difference on 25% concentration. In Escherichia coli, 75%, 50% and 25% was significantly different from the positive control and not significantly different in 100% concentration. In the negative control, there was a significant difference in 100% and 75% concentration and the other 50% and 25% was not significantly different from the negative control because of the zero inhibition result.

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