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Phytochemical screening and antimicrobial potential of hoya mindorensis methanolic crude leaf extract on staphylococcus aureus, escherichia coli, and saccharomyces cerevisiae 6

By: Bituin, Jireh V. Laihee, Kaecel Angela I. Navaja, Kim Angelo V. Ortiz, Janine E. Sablad, Patricia Anne D. 4 0 16, [, ] | [, ] |

Contributor(s): 5 6 [] |

Language: Unknown language code Summary language: Unknown language code Original language: Unknown language code Series: ; 4507846Edition: Description: 96 pagesContent type: text Media type: unmediated Carrier type: volumeISBN: ISSN: 2Other title: 6 []Uniform titles: | | Related works: 1 40 6 []Subject(s): -- 2 -- 0 -- -- | -- 2 -- 0 -- 6 -- | 2 0 -- | -- -- 20 -- | | -- -- -- -- 20 -- | -- -- -- 20 -- --Genre/Form: -- 2 -- Additional physical formats: DDC classification: | LOC classification: | | 2Other classification:

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Action note: In: Summary: ABSTRACT: Hoya mindorensis is an endemic plant in Mindoro Islands, cultivated throughout and native in the Philippines. Many Hoya species endemic in the country are not yet evaluated for their phytochemicals and antimicrobial potentials. There is a gap in current studies on the antimicrobial screening of Hoya spp. endemic in the Philippines. Hence, this study explored the phytochemicals and antimicrobial potential of H. mindorensis Schitr. methanolic leaf crude extract against common disease-causing microorganisms-Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae through disk diffusion susceptibility testing. To assess the plant's antimicrobial potential, eight concentrations were evaluated against the microorganisms prepared in 0.5 McFarland concentration. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations (MBC, MFC) were determined. Using disk diffusion susceptibility testing, the clearing zones that inhibited microbial growth were also determined. H. mindorensis possessed secondary metabolites including alkaloids, phenols and tannins, and steroids that contributed to the plant's antimicrobial activity. Flavonoids and saponins were absent. MIC screening revealed that the extract inhibited both bacterial growth at 500mg/ml (50%) and fungal growth at 250mg/ml (25%). Nonetheless, the plant had fungicidal effect on the fungal representative at 1000mg/ml (100%) considered as the MFC. Furthermore, the extract was inactive against all the microorganisms based on the disk diffusion susceptibility assay. No statistical analysis could be performed due to absence of quantifiable results. Other editions:

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Item type	Current location	Home library	Collection	Call number	Status	Date due	Barcode	Item holds
Book	PLM	PLM Filipiniana Section	Filipiniana-Thesis	QH301 B58 2023 (Browse shelf)	Available		FT8349

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Undergraduate Thesis: (Bachelor of Science in Biology) - Pamantasan ng Lungsod ng Maynila, 2023 56

ABSTRACT: Hoya mindorensis is an endemic plant in Mindoro Islands, cultivated throughout and native in the Philippines. Many Hoya species endemic in the country are not yet evaluated for their phytochemicals and antimicrobial potentials. There is a gap in current studies on the antimicrobial screening of Hoya spp. endemic in the Philippines. Hence, this study explored the phytochemicals and antimicrobial potential of H. mindorensis Schitr. methanolic leaf crude extract against common disease-causing microorganisms-Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae through disk diffusion susceptibility testing. To assess the plant's antimicrobial potential, eight concentrations were evaluated against the microorganisms prepared in 0.5 McFarland concentration. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations (MBC, MFC) were determined. Using disk diffusion susceptibility testing, the clearing zones that inhibited microbial growth were also determined. H. mindorensis possessed secondary metabolites including alkaloids, phenols and tannins, and steroids that contributed to the plant's antimicrobial activity. Flavonoids and saponins were absent. MIC screening revealed that the extract inhibited both bacterial growth at 500mg/ml (50%) and fungal growth at 250mg/ml (25%). Nonetheless, the plant had fungicidal effect on the fungal representative at 1000mg/ml (100%) considered as the MFC. Furthermore, the extract was inactive against all the microorganisms based on the disk diffusion susceptibility assay. No statistical analysis could be performed due to absence of quantifiable results.

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