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Larvicidal potential of leaf ethanolic extracts from selected mayana (Coleus scutellarioides (L.) Benth.) varieties against aedes aegypti 3rd instar larvae 6

By: Castañares, Theisa Marie Heir S. Dela Cruz, Alessandra Renee F. Lasco, Kamilah Zsofia Beatrice C. Manalad, Dean Ronald T. T. Obispado, Ma. Arriane Cea J. 4 0 16, [, ] | [, ] |

Contributor(s): 5 6 [] |

Language: Unknown language code Summary language: Unknown language code Original language: Unknown language code Series: ; 4507846Edition: Description: 132 pagesContent type: text Media type: unmediated Carrier type: volumeISBN: ISSN: 2Other title: 6 []Uniform titles: | | Related works: 1 40 6 []Subject(s): -- 2 -- 0 -- -- | -- 2 -- 0 -- 6 -- | 2 0 -- | -- -- 20 -- | | -- -- -- -- 20 -- | -- -- -- 20 -- --Genre/Form: -- 2 -- Additional physical formats: DDC classification: | LOC classification: | | 2Other classification:

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Action note: In: Summary: ABSTRACT: Because of the increasing prevalence of vector-bome resistance, and the lack of non-toxic larvicide options, the larvicidal potential of different Mayana (Coleus scutellarioides (L.) Benth.) investigated with the goal of finding a natural and accessible larvicidal alternative against Aedes egypti 3rd instar larvae. Three conveniently-sampled varieties were propagated, dried, and then subjected for ethanolic extraction to produce three different extracts, which were all further diluted into concentrations of 50 ppm, 100 ppm, and 500 ppm. Preliminary phytochemical analyses revealed the presence of flavonoids, terpenoids, and phenols for all varieties. After this, the extracts were subjected to five replicates of a larvicidal biassay procedure adapted from the World Health Organization. All varieties exhibited larvicidal activity, with Variety 1 (500 ppm) yielding the highest value of 50% larval mortality percentage after 4320 minutes. Probit analysis further supports this, as Variety 1 had a LC50 of 450 ppm, LC90 of 184,000 ppm, and LC99 of 2.6 x 107 ppm, compared to Variety 2 and 3 which both had a LC50 of 21,000 ppm, LC90 of 1.2 x 108, and LC99 of 1.5 x 1011. Furthermore, Two-way ANOVA with Replications revealed the independence of concentration and variety from each other and the significance of variety type to larval mortality. T-test assuming Equal Variances also revealed no significant difference between the most effective variety (Variety 1) and the positive control (Ocimum basilicum (L.)). Key Words: C. scutellarioides, A. aegypti, variety, concentration, mortality Other editions:

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Item type	Current location	Home library	Collection	Call number	Status	Date due	Barcode	Item holds
Book	PLM	PLM Filipiniana Section	Filipiniana-Thesis	QH671 C37 2023 (Browse shelf)	Available		FT8310

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Undergraduate Thesis: (Bachelor of Science in Biology) - Pamantasan ng Lungsod ng Maynila, 2023 56

ABSTRACT: Because of the increasing prevalence of vector-bome resistance, and the lack of non-toxic larvicide options, the larvicidal potential of different Mayana (Coleus scutellarioides (L.) Benth.) investigated with the goal of finding a natural and accessible larvicidal alternative against Aedes egypti 3rd instar larvae. Three conveniently-sampled varieties were propagated, dried, and then subjected for ethanolic extraction to produce three different extracts, which were all further diluted into concentrations of 50 ppm, 100 ppm, and 500 ppm. Preliminary phytochemical analyses revealed the presence of flavonoids, terpenoids, and phenols for all varieties. After this, the extracts were subjected to five replicates of a larvicidal biassay procedure adapted from the World Health Organization. All varieties exhibited larvicidal activity, with Variety 1 (500 ppm) yielding the highest value of 50% larval mortality percentage after 4320 minutes. Probit analysis further supports this, as Variety 1 had a LC50 of 450 ppm, LC90 of 184,000 ppm, and LC99 of 2.6 x 107 ppm, compared to Variety 2 and 3 which both had a LC50 of 21,000 ppm, LC90 of 1.2 x 108, and LC99 of 1.5 x 1011. Furthermore, Two-way ANOVA with Replications revealed the independence of concentration and variety from each other and the significance of variety type to larval mortality. T-test assuming Equal Variances also revealed no significant difference between the most effective variety (Variety 1) and the positive control (Ocimum basilicum (L.)). Key Words: C. scutellarioides, A. aegypti, variety, concentration, mortality

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