In vitro anticoagulant potential of citrus maxima (burm.) merr. suha leaf crude extract using prothrombin time assay / Samuel Brillo. 6

By: Samuel Brillo. 4 0 16, [, ] | [, ] |
Contributor(s): 5 6 [] |
Language: Unknown language code Summary language: Unknown language code Original language: Unknown language code Series: ; 201746Edition: Description: 28 cm. 76 ppContent type: text Media type: unmediated Carrier type: volumeISBN: ISSN: 2Other title: 6 []Uniform titles: | | Related works: 1 40 6 []Subject(s): -- 2 -- 0 -- -- | -- 2 -- 0 -- 6 -- | 2 0 -- | -- -- 20 -- | | -- -- -- -- 20 -- | -- -- -- 20 -- --Genre/Form: -- 2 -- Additional physical formats: DDC classification: | LOC classification: | | 2Other classification:
Contents:
Action note: In: Summary: ABSTRACT: The focus of this study is to evaluate the in vitro anticoagulant potential of Citrus maxima leaf ethanolic crude extract in human plasma using prothrombin time assay. Three concentrations were formed from the crude extract combined with normal saline solution: 10 mg/mL, 30 mg/mL and 50 mg/mL. Human platelet-poor plasma (PPP) samples were acquired from four male donors, aged 18-21. Untreated PPP samples were used as negative control to confirm normal clotting time (NCT), which is 13-16 seconds. Heparin was used as postive control. PPP samples were treated with the leaf crude extracts of different concentrations and control groups for 3 minutes and determined their clotting time in duplicate tests to record mean clotting time (MCT). Clotting time (CT) was used as parameter in measuring its anticoagulant activity. For statistical treatment, one-way ANOVA was used to determine significant difference among treatments followed by post hoc Tukey's test to identify the most effective concentration. To determine its anticoagulant potential, it was compared to positive control using independent t-test. Based on the results, all treatments exceeded the normal clotting time. There is a progressive trend in CT as the concentration increases. The highest concentration, 50 mg/ML exhibited highest CT (26.087 seconds) and prolonged CT by 77.16%. One-way ANOVA showed that all treatments were significantly different with each other. Post hoc Tukey's test revealed that the most effective concentration is 50 mg/mL. However according to t-test of heparin vs. 50 mg/mL, there was a significant difference but this implicates that the anticoagulant activity of 50 mg/mL concentration was not as potent as heparin according to t-test. Further studies can be conducted to assess the anticoagulant activity of the crude extract. Other editions:
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Thesis:(B.S. Biology)- Pamantasan ng Lungsod ng Maynila, 2017. 56

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ABSTRACT: The focus of this study is to evaluate the in vitro anticoagulant potential of Citrus maxima leaf ethanolic crude extract in human plasma using prothrombin time assay. Three concentrations were formed from the crude extract combined with normal saline solution: 10 mg/mL, 30 mg/mL and 50 mg/mL. Human platelet-poor plasma (PPP) samples were acquired from four male donors, aged 18-21. Untreated PPP samples were used as negative control to confirm normal clotting time (NCT), which is 13-16 seconds. Heparin was used as postive control. PPP samples were treated with the leaf crude extracts of different concentrations and control groups for 3 minutes and determined their clotting time in duplicate tests to record mean clotting time (MCT). Clotting time (CT) was used as parameter in measuring its anticoagulant activity. For statistical treatment, one-way ANOVA was used to determine significant difference among treatments followed by post hoc Tukey's test to identify the most effective concentration. To determine its anticoagulant potential, it was compared to positive control using independent t-test. Based on the results, all treatments exceeded the normal clotting time. There is a progressive trend in CT as the concentration increases. The highest concentration, 50 mg/ML exhibited highest CT (26.087 seconds) and prolonged CT by 77.16%. One-way ANOVA showed that all treatments were significantly different with each other. Post hoc Tukey's test revealed that the most effective concentration is 50 mg/mL. However according to t-test of heparin vs. 50 mg/mL, there was a significant difference but this implicates that the anticoagulant activity of 50 mg/mL concentration was not as potent as heparin according to t-test. Further studies can be conducted to assess the anticoagulant activity of the crude extract.

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