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Antagonistic potential of enterococcus faecalis on citrobacter freundii in vitro / Twain Pia M. Conejos. 6

By: Twain Pia M. Conejos. 4 0 16, [, ] | [, ] |

Contributor(s): 5 6 [] |

Language: Unknown language code Summary language: Unknown language code Original language: Unknown language code Series: ; March 2017.46Edition: Description: 28 cm. 64 ppContent type: text Media type: unmediated Carrier type: volumeISBN: ISSN: 2Other title: 6 []Uniform titles: | | Related works: 1 40 6 []Subject(s): -- 2 -- 0 -- -- | -- 2 -- 0 -- 6 -- | 2 0 -- | -- -- 20 -- | | -- -- -- -- 20 -- | -- -- -- 20 -- --Genre/Form: -- 2 -- Additional physical formats: DDC classification: | LOC classification: | | 2Other classification:

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Action note: In: Summary: ABSTRACT: The study aimed to determine the antagonistic potential of Enterococcus faecalis on Citrobacter freundil. In order to determine the antagonistic potential of E. Faecalis, disk diffusion assay was done. The assay was done in triplicates. In order to confirm the presumptions, a confirmatory assay for the MIC was done. Minimum Bactericidal Concentration (MBC) followed after streaking the contents of the wells used in the MIC. The Minimum Inhibitory Concentration (MIC) of the E. Faecalis was obtained using a two-fold dilution process. After 24 hours, the plates were observed. Both the positive control (Cefepime) and the experimental group (E. Faecalis) showed antagonistic activity while the NSS did not inhibit the growth of C. freundili at all. An average zone of inhibition equivalent to 26.2 mm (positive control) and 21.21 mm (experimental group were observed and this clearing zone was categorized as active. For the qualitative tests. It was observed that after 24 hours of incubation, well no. 9 was recorded as the MIC as no visible turbidity of the well was observed. It has been confirmed that the presumed concentration 9 was the MIC. The presence of significant difference among the zones of inhibition was analyzed using two tail T-test (alpha = 0.05). The prepared inoculum of E. Faecalis, using the Mc Farland Standard as basis for initial and accurate no. of bacterial population, did not show a significant difference in terms of ZOI measurement between the positive control Cefepime and the experimental group/control. The results show that E. faecalis have components/specific biology that enabled a close antagonistic performance with the positive control. Other editions:

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Item type	Current location	Home library	Collection	Call number	Status	Date due	Barcode	Item holds
Book	PLM	PLM Filipiniana Section	Filipiniana-Thesis	T QR82.C66.2017 (Browse shelf)	Available		FT6246

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Thesis:(B.S. Biology)- Pamantasan ng Lungsod ng Maynila, 2017. 56

ABSTRACT: The study aimed to determine the antagonistic potential of Enterococcus faecalis on Citrobacter freundil. In order to determine the antagonistic potential of E. Faecalis, disk diffusion assay was done. The assay was done in triplicates. In order to confirm the presumptions, a confirmatory assay for the MIC was done. Minimum Bactericidal Concentration (MBC) followed after streaking the contents of the wells used in the MIC. The Minimum Inhibitory Concentration (MIC) of the E. Faecalis was obtained using a two-fold dilution process. After 24 hours, the plates were observed. Both the positive control (Cefepime) and the experimental group (E. Faecalis) showed antagonistic activity while the NSS did not inhibit the growth of C. freundili at all. An average zone of inhibition equivalent to 26.2 mm (positive control) and 21.21 mm (experimental group were observed and this clearing zone was categorized as active. For the qualitative tests. It was observed that after 24 hours of incubation, well no. 9 was recorded as the MIC as no visible turbidity of the well was observed. It has been confirmed that the presumed concentration 9 was the MIC. The presence of significant difference among the zones of inhibition was analyzed using two tail T-test (alpha = 0.05). The prepared inoculum of E. Faecalis, using the Mc Farland Standard as basis for initial and accurate no. of bacterial population, did not show a significant difference in terms of ZOI measurement between the positive control Cefepime and the experimental group/control. The results show that E. faecalis have components/specific biology that enabled a close antagonistic performance with the positive control.

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