Prevalence of trypanosoma evansi in cattle (bos taurus) from Maragondon and Magallanes, Cavite through blood smear technique / 6
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Rizza E. Buenavidez.
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- 65 pp. 28 cm.
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Thesis:(B.S Biology)- Pamantasan ng Lungsod ng Maynila, 2017.
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ABSTRACT: The present study was conducted to know the prevalence rate of the Trypanosoma evansi in the two chosen municipalities, Maragondon and Magallanes, Cavite. Also, the goal of this study was to know the significant difference between prevalence rates of Trypanosoma evansi in the two municipalities. Consent form was presented to cattle owners during blood collection, Blood was collected from the peripheral vein in the ear of the cattle. Fixation of thin blood smears was immediately done using metanol. Giemsa Stain was used in staining the blood smears. The total sample size was 38 cattle in each municipality. A total of 152 blood smears were subjected to microscopic examination (1000x) in detecting the presence of Trypanosoma evansi. After several microscopic observations, however, there was no presence of Trypanosoma evansi in all of the slides examined. This negative result suggests that there is a zero prevalence rate in Maragondon and Magallanes. Since there are no prevalence rates that can be used for comparison, the statistical analysis was not performed. The negative result might be due to low sensitivity of diagnostic method used and low sample size.