Fabian, Ray Chester Nogales.  4  0   <br/><br/>
Sorption and Morphological Effects of Heavy Metal lons on Luminescent Bacteria Vibrio harveyi /        6 
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Fabian, Ray Chester Nogales. 
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<br/><br/>   Thesis: (B.S. Biology) - Pamantasan ng Lungsod ng Maynila, 2005. 
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<br/><br/> ABSTRACT:  	The study was conducted in order to determine sorption rate and growth rate of Vibrio harveyi when subjected to different concentrations (1, 5, 10, 15, 30ppm) of manganese (II), copper (II) and zinc (II) ion. Bioindicator potential of the test bacteria was also considered.  	BOSS media was used to culture the bacteria and asseptic technique was used to prevent contamination of other bacteria. Heavy metal ion were introducede to the media and a two-hour interval was observed during the assay. The samples were centrifuged to extract the bacterial pellet. Supernatant was subjected to atomic absorption spectrophotometry (AAS)n for the study of sorption kinetics while the dried bacterial pellets were weighed together with the pre-weighed test tubes. After the heavy metal assay, bioluminescence property of the bacteria was observed in a dark room. Statistical analysis of the data were analyzed using one-way, two-way ANOVA, Dunnett's test, Turkey's test and correlation.  	A decreased in heavy metal ion content was observed with the three heavy metals used in the study. However there were no significant difference among the varying concentration and varying heavy metal ions used in terms of growth and sorption kinetics. Significant difference was only observed in the percentage of metal sorbed by the bacteria. Copper ion was significantly different from the other two heavy metal ions. All concentrations of the three heavy metal ions, Mn(II), Cu(II) and Zn(II), used in the study did not inhibit the luminescence property of the bacteria.       
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