Optimization of Chlorophyll a Production of Some Cyanobacteria from Rice Paddies in Manipur, India Through Nutritional and Environmental Factors 6
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ABSTRACT: Experiments were carried out to examine the production of chlorophyll a by cyanobacteria against various concentrations of nitrate, phosphate, pH and light qualities. It was observed that highest chlorophyll a was produced by Anabaena spiroides in 0N concentration of sodium nitrate followed by Phormidium arthurensis in 1½N concentration of sodium nitrate during 30th day of growth. Pertaining to the effect of phosphate, maximum chlorophyll a production was observed by Nostoc piscinale in 1½N followed by Nostoc muscorum in 2N concentration of dipotassium hydrogen phosphate during 30th day of growth. Photochromatic adaptation studies revealed that maximum chlorophyll a production was observed in Nostoc muscorum in white light (31.10 µg g-1) followed by red light (14.70 µg g-1) and blue light (8.53 µg g-1) during 30th day of growth. In green light, chlorophyll a production was minimum (4.85 µg g-1) during 30th day of growth. The strain Nostoc muscorum produced maximum chlorophyll a production at pH 8.0 (26.9 29 µg g-1), whereas Nostoc piscinale yielded maximum chlorophyll a at pH 8.5 (26.30 µg g-1). Nostoc muscorum showed comparatively higher chlorophyll a content at all pH values.