TY - BOOK AU - AU - Bemboy Nino F. Subosa ED - ED - ED - Philippine Engineering Journal. 37:1 (June 2016): pp.63-78 ED - SN - 2 PY - 0000///46 CY - PB - KW - KW - 2 KW - 0 KW - 6 KW - 20 KW - Peptone Production KW - Nutrient Media KW - sears0 N1 - ABSTRACT : Peptones are the product of a protein hydrolysis, which serve as the main nutrient source for bacteria in a culture media. This study aimed to provide a locally feasible process for peptone production. Optimization results for Phase 1 showed that a temperature of 90C, a digestion time of 4 hours and a ratio 0.06g feathers/mL produced a yield of 50.6%. Statistical analysis showed that E. coli growth on the laboratory - produced peptone is significantly greater than the growth realized on commercial peptone and plate count agar. Purification, dying and characterization techniques were integrated into the existing process for Phase 2 to obtain peptones with better commercial quality. The maximum yield obtained was 30 g peptone per 100 g feathers. Freeze-dried powder from the purified hydrolysates had reduced odor and moisture as compared to the vacuum dried peptone . The vacuum-filtered batch also approximated the physical characteristics of the standard peptone hyfrolysate. Performance testing for Phase 2 showed increased support for bacterial growth; 5 ER -