Phytochemical and microbiological screening of the Lipote (Syzglum polycephaloides) leaf extract : an experiment in microbiology / Chona G. Tibayan. 6

Thesis (M.A.)-- Pamantasan ng Lungsod ng Maynila, 1998.;A thesis presented to the faculty of the Graduate School of Arts, Sciences, Education and Nursing in partial fulfillment of the requirements for the degree Master of Arts in Education, Biological Science. 56

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ABSTRACT: SPECIFIC OBJECTIVES : 1. to determine the chemical constituents of the leaf extract and the effect of the extract on test organisms using varying concentration of test stample. 2. to develop laboratory activities arising from the phytochemical and antimicrobial analysis of the leaf extract. PROCEDURE: Phase I Phytochemical Analysis of Lipote (Syzygium polycephaloides ) leaf extract Phase II Antimicrobial Analysis of the leaf extract using varied concentration of test samples Phase III Development of laboratory activities arising from Phase I and Phase II of the study. FINDINGS: 1. The 80% ethyl alcohol leaf extract contains flavonoids sterols, tannins, cyaniding, carbohydrates and proteins. Alkaloids and saponins were absent in the extract. Inroganic salt such as chloride, sulfate, sodium and potassium were likewise present. 2. In the microbial screening, the leaf extract exhibited antimicrobial effect against S. aureus and E. coli. The mean inhibitions for 50%, 75% and 100% concentrations were 10.075 mm, 10.625 mm and 11.25 mm compared with the inhibitions produced by using Micanazole Nitrate which were 9.9 mm, 10.50 mm and 12.00 mm using the same concentrations. The zone inhibitions produced in E. coli using 50%, 75%, 100% concentrations were 10.50 mm, 14.75 mm and 28 mm while the zones of inhibition produced by the standard. Miconazole Nitrate using the same concentration 12.25 mm, 14.00 mm and 29.00 mm. The lipote extract did not show any inhibition on Candida albicans and Trichopolyton rubrum. 3. The 100% concentration of the leaf extract exhibited the widest mean of growth inhibition in both S. aureus and E. coli with a result of 11.25 mm and 28 mm respectively and the narrowest mean of growth inhibition at 50% concentration, 10.0075 mm for S. aureus and 10.50 mm for E. coli. CONCLUSION: Lipote leaf extract contains active and inactive constituents, which can be used for medicinal purpose. The leaf extract showed antibacterial properly and therefore can be used as an antibacterial agent. The developed laboratory activities can be used by Microbiology laboratory instructors in enhancing students skills in Microbiology. RECOMMENDATION: The researcher recommends that further research be conducted on the isolation, purification and chemical investigation of the constituents that is responsible for the antibacterial activity. Pharmacological screening and toxicity studies should be done for future medicinal use. Furthermore, the result of these study can be used by Microbiology teachers in enhancing the manipulative and conceptual skills of students in Microbiology. The researcher hopes that the result of the study may serve to motivate young researchers to conduct investigations to obtain products thru forms of investigatory projects. Thus, they will be able to develop creative and technological skills.

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