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_aBituin, Jireh V. Laihee, Kaecel Angela I. Navaja, Kim Angelo V. Ortiz, Janine E. Sablad, Patricia Anne D.
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_aPhytochemical screening and antimicrobial potential of hoya mindorensis methanolic crude leaf extract on staphylococcus aureus, escherichia coli, and saccharomyces cerevisiae
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_c4507846
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_a96 pages
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_atext
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_aunmediated
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_aUndergraduate Thesis: (Bachelor of Science in Biology) - Pamantasan ng Lungsod ng Maynila, 2023
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_aABSTRACT: Hoya mindorensis is an endemic plant in Mindoro Islands, cultivated throughout and native in the Philippines. Many Hoya species endemic in the country are not yet evaluated for their phytochemicals and antimicrobial potentials. There is a gap in current studies on the antimicrobial screening of Hoya spp. endemic in the Philippines. Hence, this study explored the phytochemicals and antimicrobial potential of H. mindorensis Schitr. methanolic leaf crude extract against common disease-causing microorganisms-Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae through disk diffusion susceptibility testing. To assess the plant's antimicrobial potential, eight concentrations were evaluated against the microorganisms prepared in 0.5 McFarland concentration. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations (MBC, MFC) were determined. Using disk diffusion susceptibility testing, the clearing zones that inhibited microbial growth were also determined. H. mindorensis possessed secondary metabolites including alkaloids, phenols and tannins, and steroids that contributed to the plant's antimicrobial activity. Flavonoids and saponins were absent. MIC screening revealed that the extract inhibited both bacterial growth at 500mg/ml (50%) and fungal growth at 250mg/ml (25%). Nonetheless, the plant had fungicidal effect on the fungal representative at 1000mg/ml (100%) considered as the MFC. Furthermore, the extract was inactive against all the microorganisms based on the disk diffusion susceptibility assay. No statistical analysis could be performed due to absence of quantifiable results.
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