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_aAidelenn Tacadena Wong.
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_aIsolation of lipids and characterization of fatty acid methyl ester from mucor subtilissimus /
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_cAidelenn Tacadena Wong.
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_cMarch 2002.46
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_a57 pp.
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_aThesis: (Bachelor of Science in Chemistry) - Pamantasan ng Lungsod ng Maynila, 2002.
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_aABSTRACT: This study entitled Isolation of Lipids and Characterization of Fatty Acid Methyl Ester from Mucor subtilissimus aimed to identify which of the locally cultured media was the best media for the production of biomass and oil for Mucor subtilissimus. Purpose of this study was also to determine what were the components of the oil. This was done through the extraction oil from fungi with different kinds of locally cultured media, using organic solvents like chloroform, hexane, ethanol and methanol. Gas chromatography method was used for the identification of fatty acid distribution. Based on the statistical treatments used it was found out that there was significant difference between the biomass of Mucor subtilissimus using the three locally formulated media. It was best lived on Banana Sugar Media followed by Melon Sugar Media and least lived on Coconut Water Sugar Media. It was also found out that there was a significant difference between the productions of oil of Mucor subtilissimus grown in different media. Oil was abundant in fungi grown in Banana Sugar Media followed by Melon Sugar Media and least in Coconut Water Sugar Media. After the determination of the best media for the growth and production of oil in Mucor subtilissimus it was then subjected into identification of the fatty acid content of the soil. The analysis reported that 24.8% of the oil was component A, based on the researcher's identification through patterns of time retention and elution, it is concluded as Caprylic Acid. Produced in the body in small amounts, antifungal in human sweat and sebum, natural component of coconut oil, palm nut oil, butter fat and other vegetable and animal sources, synthesized from caprylic alcohol (octanol) found in coconut oil.
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