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_aKristine May G. De Asis.
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_aAntifungal potential of tungkod-pare (cordyline fruticosa) crude leaf extract on fusarium solani /
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_cKristine May G. De Asis.
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_cMarch 2017.46
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_a48 pp.
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_aThesis:(B.S. Biology)- Pamantasan ng Lungsod ng Maynila, 2017.
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_aABSTRACT: The objective of this research is to determine the antifungal potential of Tungkod-pare (cordyline fruticosa) crude leaf extract on Fusarium solani. The leaves of Cordyline fruticosa and the positve control, Annona squamosa, were collected, washed and extracted using methanol. The crude extract was obtained using rotary evaporator. Furthermore, qualitative phytochemical analysis was done in order to determine the presence of different biological compounds. The different concentrations (1000 mg/mL, 500 mg/mL, 250 mg/mL, 125 mg/mL, 62.5 mg/mL, 31.3 mg/mL, 15.6 mg/mL, 7.8 mg/mL) were used in the determination of Minimal Inhibitory Concentration (MIC) and the results were confirmed by the Minimal Fungicidal Concentration (MFC). Paper Disc Diffusion was also performed for the determination of Zone of Inhibition (ZOI). The assays were done in triplicates. There was no zone of inhibition (ZOI) observed in the positive control, as well as in Cordyline fruticosa. It was observed in both plants that resistant strains were present in the highest concentration used. No statistical analysis was utilized since negative results were obtained . Therefore, Tungkod-pare (Cordyline fruticosa) crude leaf extract did not exhibit antifungal activity on Fusarium solani.
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