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_aChislev Noellee B. Cortez.
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_aPrevalence of trypanosoma evansi infection in cattle (bos taurus) from Tagaytay and Silang, Cavite using blood smear technique /
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_cChislev Noellee B. Cortez.
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_cMarch 2017.46
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_a54 pp.
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_aThesis:(B.S Biology)- Pamantasan ng Lungsod ng Maynila, 2017.
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_aABSTRACT The parasite trypanosoma evansi locally known as surra in the Philippines causes mortality and weak productivity in domestic animals such as cattle which would affect the livelihood and production of meat and dairy products of cattle owners. This study was conducted to detect the presence and identify the prevalence of trypanosoma evansi infection in the cattle of Silang and Tagaytay Cavite using Blood Smear technique. Different ages and both genders of the cattle were used for blood collection 208 cattle were sampled in Tagaytay City and 112 cattle were sampled in Silang Cavite. Using a sterile needle, blood was collected by puncturing the peripheral vein of the ear of the cattle. The fresh blood was smeared through thick and thin smears then air dried. Thin smears were fixed with methanol. both smears were stained with Giemsa Stain for 25 minutes and washed after drying. The slides with blood smears were examined under the microscope through HPO (400x) and OIO (1000x) No Statistical analysis was performed due to zero percent prevalence of Trypanosoma evansi in the study sites. Out of 108 cattle sampled in Tagaytay City 112 cattle sampled in Silang Cavite, the cattle sampled were not contracting Surra infection based on the results of blood smear examinations. This signifies that the areas are still safe for the cattle to occupy. The research would like to recommend using sensitive techniques and devices in detecting Trypanosoma evansi like polymerase Chain Reaction (PCR) Card Agglitination Test (CATT) and Mouse Inoculating Test (MIT) especially in tagaytay City and Silang Cavite.
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