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_aJanella G. Redrino.
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_aBacterial analysis of selected sandwich spreads sold in a local market in Manila /
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_cJanella G. Redrino.
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_cMarch 2017.46
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_a73 pp.
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_aThesis:(B.S. Biology) - Pamantasan ng Lungsod ng Maynila, 2017.
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_aABSTRACT: The main objective of this study is to analyze the bacterial content on selected sandwich spreads (peanut butter, chicken spread, and chocolate spread) sold in a local market in Manila and to determine the risk factors associated with the bacterial contamination in the sandwich spread samples. Four stalls were the source of the tree sandswich spread samples. Each store was assesed thru the prepared risk factor evaluation form. Sample homogenate was aseptically prepared and analyzed for the aerobic plate count, enumeration of coliforms and Escherichia coli and isolation of Salmonella. Gram staining and seven biochemical testing were undertaken to give the presumptive identity of isolated colonies. A total of 19 isolates belonging to 6 genera were identified: Serratia, Providencia, Enterobacter, Escherichia, Salmonella and Edwardsiella. In peanut butter and chocolate spread, Serratia sp. Had the highest percentage of occurrence, 50% and 33%, respectively. Whereas, Serratia sp. And Enterobacter sp. Were the most commonly found genera isolate detected with 40% occurrence each. Peanut butter had the highest CFU/ml with an average of 2.6 x 10 CFU/ml. Followed by a chicken spread with an of 1.86x10 CFU/ml. Chocolate spread had an average of 1.7x10 CFU/ml. The bacterial count among the three selected sandwich spread samples showed no signifficant difference in terms of CFU/ml. Factors related to high bacterial densities were an unapproved source of products. Containers lids that were not tightly fitted; unrefrigerated products and store's area had insects and rodents infestation. All three-sandwich spread has exceeded and failed to meet the standard bacterial count implemented by FDA. Also, the occurrence of E. Coli and salmonella sp. Implies the likelihood of a microbial hazard and fecal contamination. Thus, the data obtained in this study indicate taht the selected sandwich spreads were unsafe and could be a possible source of foodborne illness and must be addressed and monitored by the authorities.
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