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050 _aQD415 A53 2025
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100 1 _a Andres, Kritz Marie M.; Janobas, Levi Jahziel G.; Marcelino, Arabella S.; Nueva, Shekinah Mae B.
245 _aPhytochemicals screening, antioxidant activity, and cytotoxicity of four Philippine endemic nepenthes species
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300 _bUndergraduate Thesis: (Bachelor of Science in Chemistry) - Pamantasan ng Lungsod ng Maynila, 2025
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505 _aABSTRACT: A study was conducted on four endemic Nepenthes species of the Philippines (N. graciliflora, N. mindanaoensis, N. mirabilis, N. ventricose) to test their phytochemical content, antioxidant activity, and cytotoxicity. The samples were all positive for alkaloids, flavonoids, saponins, tannins, and terpenoids, except for N. ventricose, which was negative for saponins and tannins, and N. mirabilis, which was negative for terpenoids. In terms of their antioxidant activity, the samples are less effective than ascorbic acid. However, N. mirabilis and N. mindanaoensis produced relatively high percentage inhibition with 84.4% and 95.12% effectivity, respectively, while N. ventricose and N. Graciliflora have low percentage inhibition with 40.7% and 26.72%, respectively. Statistical tests showed that generally, they differ significantly, but there is no notable difference when compared individually. Based on their mg GAE/g extract, N. mindanaoensis has the highest total phenolic content (TPC) with the value of 291.667 = 14.556, followed by N. mirabilis and N. ventricose, resulting in 149.030 = 4.920 and 123.425 = 1.198, respectively, while N. graciliflora having the lowest TPC of 49.0917 = 1.700. Based on their calculated L.C50, N. graciliflora and N. ventricose are both non-toxic, N. mindanaoensis is non-toxic to low toxic. However, N. mirabilis is considered medium toxic to toxic.
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