Charlotte F. Marasigan. 4 0

Radioprotective potential of leea manillensis syn. Guineensis (abang-abang) leaf extract on gamma-irradiated human blood lymphocytesn in vitro / 6 6 Charlotte F. Marasigan. - - - 31 pp. 28 cm. - - - - - . - . - 0 . - . - 0 .

Thesis:(B.S. Biology)-Pamantasan ng Lungsod ng Maynila, 2017.





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ABSTRACT: The objective of the study was to evaluate the Radioprotective Potential of Leea manillensis syn. guineensis (Abang-abang) extract on gamma irradiated human lymphocytes in vitro using the micronucleus assay. The 100% pure concentration of Leea manillensis syn. guineensis (Abang-abang) extract was diluted into varying concentration of 0.1 mg/ml, 0.2 mg/ml, 0.3 mg/ml, and 0.4 mg/ml which were added to the irradiated blood samples. Based on the experiment, the extract of Leea manillensis syn, guineensis (Abang-abang) reduced the number of the micronuclei in the 1000 binucleated cells. Hoewever only 0.4 mg/ml of Leea manillensis syn. guineenis (Abang-abang) exhibited the greatest radioprotective activity in terms of the reduced micronucleus formation. One-way analysis of variance (ANOVA) was utilized to determine and evaluate if there is a significant difference between the experimental group. It demonstrated that the Leea manillensis syn. guineesis (Abang-abang) extract reduced the number of micronuclei and the highest plant concentration which was 0.4 mg/ml yielded the least number of micronuclei which was 0.4 mg/ml yielded the least number of micronuclei which scored a mean of 33. Furthermore, it was observed and evident that the 0.4 mg/ml plant concentration had no significant difference (p<0.05) from the positive control, oregano extract.













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